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peptivator ebv ebna 1 peptide pool  (Miltenyi Biotec)


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    Miltenyi Biotec peptivator ebv ebna 1 peptide pool
    Peptivator Ebv Ebna 1 Peptide Pool, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/peptide+pools/PepTivator+EBV+EBNA-1%2C+premium+grade/pm42518308-45-31-36
    Average 93 stars, based on 10 article reviews
    peptivator ebv ebna 1 peptide pool - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Minimal association between Th1-specific responses to COVID-19 vaccines and SARS-CoV-2 breakthrough infections in multiple sclerosis patients receiving disease-modifying therapies
    Article Snippet: Specifically, 600 μL of blood was stimulated in a 48-well plate with peptide pools covering the spike protein sequence derived from SARS-CoV-2 Wuhan-Hu-1 (Wuhan spike) and the Delta variant (Delta spike). .. The Wuhan spike pool consisted of equal amounts of three peptide pools (PepTivator ® Prot_S1, Prot_S, and Prot_S+, Miltenyi Biotec, Bergisch Gladbach, Germany, Cat. 130–127–048, Cat. 130–126–701, and Cat. 130–127–312) used at a final concentration of 0.1 μg/mL. ..

    Article Title: Clonal structure and the specificity of vaccine-induced T cell response to SARS-CoV-2 Spike protein
    Article Snippet: .. Freshly isolated PBMCs were plated in a 96-well plate (Sarstedt; 83.3925.500) at a density of 1.5 × 10 6 cells/well in RPMI 1640 culture medium (Thermo; 31870025) supplemented with 1 mM sodium pyruvate (Thermo; 11360039), 5% normal human A/B serum, 1 mM L-glutamine (Thermo; 25030-024) and SARS-CoV-2 S protein-derived peptide pools (Miltenyi Biotec; 130-126-701) (final concentration 1 mM) followed by 1h incubation 37°C, 5% СО2. .. GolgiPlug (BD Biosciences; 555028) was added for 5h incubation (37°С, 5% СО2).

    Variant Assay:

    Article Title: Polyfunctional CD4 T-cells correlating with neutralising antibody is a hallmark of COVISHIELD TM and COVAXIN ® induced immunity in COVID-19 exposed Indians.
    Article Snippet: Cells were stimulated for 20 h at 37 °C with Peptivator peptide pools (Miltenyi Biotec) spanning the entire sequence of SARS-CoV-2 structural proteins, i.e., complete spike (S), nucleocapsid (N) or membrane (M) at a final concentration of 1 μg/ml and costimulatory antibodies CD28/49d at 1 μg/ml. .. In selected experiments, cells were also stimulated with the Delta variant (B.1.617.2), Omicron (B.1.1.529) BA.1 and BA.2 variants and their matched wildtype peptide pools (Miltenyi Biotec; Delta: 130–128–761 and 763, Omicron BA.1: 130–129–927 and 928 and BA.2: 130–130–806 and 807). ..

    Isolation:

    Article Title: Tracking the immune response profiles elicited by the BNT162b2 vaccine in COVID-19 unexperienced and experienced individuals.
    Article Snippet: Multiple vaccines have been approved to control COVID-19 pandemic, with Pfizer/BioNTech (BNT162b2) being

    Article Title: Clonal structure and the specificity of vaccine-induced T cell response to SARS-CoV-2 Spike protein
    Article Snippet: .. Freshly isolated PBMCs were plated in a 96-well plate (Sarstedt; 83.3925.500) at a density of 1.5 × 10 6 cells/well in RPMI 1640 culture medium (Thermo; 31870025) supplemented with 1 mM sodium pyruvate (Thermo; 11360039), 5% normal human A/B serum, 1 mM L-glutamine (Thermo; 25030-024) and SARS-CoV-2 S protein-derived peptide pools (Miltenyi Biotec; 130-126-701) (final concentration 1 mM) followed by 1h incubation 37°C, 5% СО2. .. GolgiPlug (BD Biosciences; 555028) was added for 5h incubation (37°С, 5% СО2).

    Irradiation:

    Article Title: Tracking the immune response profiles elicited by the BNT162b2 vaccine in COVID-19 unexperienced and experienced individuals.
    Article Snippet: Multiple vaccines have been approved to control COVID-19 pandemic, with Pfizer/BioNTech (BNT162b2) being

    Incubation:

    Article Title: Tracking the immune response profiles elicited by the BNT162b2 vaccine in COVID-19 unexperienced and experienced individuals.
    Article Snippet: Multiple vaccines have been approved to control COVID-19 pandemic, with Pfizer/BioNTech (BNT162b2) being

    Article Title: Clonal structure and the specificity of vaccine-induced T cell response to SARS-CoV-2 Spike protein
    Article Snippet: .. Freshly isolated PBMCs were plated in a 96-well plate (Sarstedt; 83.3925.500) at a density of 1.5 × 10 6 cells/well in RPMI 1640 culture medium (Thermo; 31870025) supplemented with 1 mM sodium pyruvate (Thermo; 11360039), 5% normal human A/B serum, 1 mM L-glutamine (Thermo; 25030-024) and SARS-CoV-2 S protein-derived peptide pools (Miltenyi Biotec; 130-126-701) (final concentration 1 mM) followed by 1h incubation 37°C, 5% СО2. .. GolgiPlug (BD Biosciences; 555028) was added for 5h incubation (37°С, 5% СО2).



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    Comparison of naïve and COVID-19 convalescent donor T cell activation for select AIM pairs. ( a ) Flow cytometry plots comparing naïve and COVD-19 convalescent donor PBMCs with <t>or</t> <t>without</t> <t>SARS-CoV-2</t> peptide pool stimulation . Five CD4 + T cell ( b ) and five CD8 + T cell ( c ) AIM pairs discriminate naïve (circle) and COVID-19 convalescent (square) donors. ( b , c ) Graphs indicate significant differential expression between naïve and COVID-19 convalescent donors, at 5% FDR (*), ns = not significant.
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    Image Search Results


    Comparison of naïve and COVID-19 convalescent donor T cell activation for select AIM pairs. ( a ) Flow cytometry plots comparing naïve and COVD-19 convalescent donor PBMCs with or without SARS-CoV-2 peptide pool stimulation . Five CD4 + T cell ( b ) and five CD8 + T cell ( c ) AIM pairs discriminate naïve (circle) and COVID-19 convalescent (square) donors. ( b , c ) Graphs indicate significant differential expression between naïve and COVID-19 convalescent donors, at 5% FDR (*), ns = not significant.

    Journal: Scientific Reports

    Article Title: Prediction of SARS-CoV-2 exposure through T cell activation profiles

    doi: 10.1038/s41598-026-48323-7

    Figure Lengend Snippet: Comparison of naïve and COVID-19 convalescent donor T cell activation for select AIM pairs. ( a ) Flow cytometry plots comparing naïve and COVD-19 convalescent donor PBMCs with or without SARS-CoV-2 peptide pool stimulation . Five CD4 + T cell ( b ) and five CD8 + T cell ( c ) AIM pairs discriminate naïve (circle) and COVID-19 convalescent (square) donors. ( b , c ) Graphs indicate significant differential expression between naïve and COVID-19 convalescent donors, at 5% FDR (*), ns = not significant.

    Article Snippet: Curated SARS-CoV-2 peptide pools (130-127-309, Miltenyi and 3622-1, Mabtech) containing 9-22 mer peptides were used to activate SARS-CoV-2 antigen-responsive CD4 + and CD8 + T cells.

    Techniques: Comparison, Activation Assay, Flow Cytometry, Quantitative Proteomics

    Comparison of naïve and COVID-19 convalescent donor T cell activation using fold-change and difference analyses. ( a ) Difference analysis for AIM pairs identified from the CD4 + T cell population; wherein, the median background subtracted activation marker pair signal from the naïve donor samples was subtracted from the background subtracted activation AIM pair signals detected from each COVID convalescent donor sample. ( b ) Fold-change analysis for marker pairs identified from the CD4 + T cell population; wherein, the background subtracted activation AIM pair signals detected from each COVID convalescent donor sample were divided by the median background subtracted activation marker pair signal from the naïve donor samples. ( c ) Difference analysis for AIM pairs identified from the CD8 + T cell population, analyzed similarly to the CD4 + T cell population. ( d ) Fold-change analysis for marker pairs identified from the CD8 + T cell population, analyzed similarly to the CD4 + T cell population. Each data point represents cells from a single COVID-19 convalescent donor treated with a single SARS-CoV-2 peptide pool in comparison to the median signal from the naïve donors. For all graphs, each unique symbol color represents data from a unique COVID-19 convalescent donor.

    Journal: Scientific Reports

    Article Title: Prediction of SARS-CoV-2 exposure through T cell activation profiles

    doi: 10.1038/s41598-026-48323-7

    Figure Lengend Snippet: Comparison of naïve and COVID-19 convalescent donor T cell activation using fold-change and difference analyses. ( a ) Difference analysis for AIM pairs identified from the CD4 + T cell population; wherein, the median background subtracted activation marker pair signal from the naïve donor samples was subtracted from the background subtracted activation AIM pair signals detected from each COVID convalescent donor sample. ( b ) Fold-change analysis for marker pairs identified from the CD4 + T cell population; wherein, the background subtracted activation AIM pair signals detected from each COVID convalescent donor sample were divided by the median background subtracted activation marker pair signal from the naïve donor samples. ( c ) Difference analysis for AIM pairs identified from the CD8 + T cell population, analyzed similarly to the CD4 + T cell population. ( d ) Fold-change analysis for marker pairs identified from the CD8 + T cell population, analyzed similarly to the CD4 + T cell population. Each data point represents cells from a single COVID-19 convalescent donor treated with a single SARS-CoV-2 peptide pool in comparison to the median signal from the naïve donors. For all graphs, each unique symbol color represents data from a unique COVID-19 convalescent donor.

    Article Snippet: Curated SARS-CoV-2 peptide pools (130-127-309, Miltenyi and 3622-1, Mabtech) containing 9-22 mer peptides were used to activate SARS-CoV-2 antigen-responsive CD4 + and CD8 + T cells.

    Techniques: Comparison, Activation Assay, Marker

    Cytokine production is increased in COVID-19 convalescent donors following stimulation with SARS-CoV-2 peptide pools. ( a ) IFN-γ cytokine production is increased in COVID-19 convalescent donor samples following stimulation with SARS-CoV-2 pp B as monitored via an Enzymatic 2-step ELISpot assay. Shown are a graph and a representative image of ELISpot results. Note: due to a technical issue, pp A failed to elicit an ELISpot response in 2 experiments, even for positive controls, which impacted the naïve to COVID-19 convalescent comparison for the pp A ELISpot data. ( b ) IFN-γ, IL-2, IL-6, and IL-10 cytokine production is increased in COVID-19 convalescent donor samples following SARS-CoV-2 peptide stimulation as monitored via a bead-based flowcytometry assay. ( a, b ) Graphs indicate significant differential expression between naïve and COVID-19 convalescent donors, at 5% FDR (*), ns = not significant. ( c ) Spearman correlation analysis of AIM pairs described in Fig. that significantly discriminated naïve and COVID-19 convalescent samples with cytokine release assay and ELISpot.

    Journal: Scientific Reports

    Article Title: Prediction of SARS-CoV-2 exposure through T cell activation profiles

    doi: 10.1038/s41598-026-48323-7

    Figure Lengend Snippet: Cytokine production is increased in COVID-19 convalescent donors following stimulation with SARS-CoV-2 peptide pools. ( a ) IFN-γ cytokine production is increased in COVID-19 convalescent donor samples following stimulation with SARS-CoV-2 pp B as monitored via an Enzymatic 2-step ELISpot assay. Shown are a graph and a representative image of ELISpot results. Note: due to a technical issue, pp A failed to elicit an ELISpot response in 2 experiments, even for positive controls, which impacted the naïve to COVID-19 convalescent comparison for the pp A ELISpot data. ( b ) IFN-γ, IL-2, IL-6, and IL-10 cytokine production is increased in COVID-19 convalescent donor samples following SARS-CoV-2 peptide stimulation as monitored via a bead-based flowcytometry assay. ( a, b ) Graphs indicate significant differential expression between naïve and COVID-19 convalescent donors, at 5% FDR (*), ns = not significant. ( c ) Spearman correlation analysis of AIM pairs described in Fig. that significantly discriminated naïve and COVID-19 convalescent samples with cytokine release assay and ELISpot.

    Article Snippet: Curated SARS-CoV-2 peptide pools (130-127-309, Miltenyi and 3622-1, Mabtech) containing 9-22 mer peptides were used to activate SARS-CoV-2 antigen-responsive CD4 + and CD8 + T cells.

    Techniques: Enzyme-linked Immunospot, Comparison, Quantitative Proteomics, Release Assay

    Unsupervised hierarchical clustering of all 35 CD4 + and CD8 + T cell AIM pair combinations. Using data from normalized samples, unsupervised hierarchical clustering of all 35 CD4 + and CD8 + T cell marker pair combinations separated naïve and COVID-19 convalescent donor samples, except for a single COVID-19 convalescent donor sample stimulated with SARS-CoV-2 pp A (blue arrow). The same donor sample stimulated with SARS-CoV-2 pp B clustered with the COVID-19 convalescent donor samples. Obtaining this clear separation of naïve and convalescent donor samples by chance is very unlikely, p < 2 -10 .

    Journal: Scientific Reports

    Article Title: Prediction of SARS-CoV-2 exposure through T cell activation profiles

    doi: 10.1038/s41598-026-48323-7

    Figure Lengend Snippet: Unsupervised hierarchical clustering of all 35 CD4 + and CD8 + T cell AIM pair combinations. Using data from normalized samples, unsupervised hierarchical clustering of all 35 CD4 + and CD8 + T cell marker pair combinations separated naïve and COVID-19 convalescent donor samples, except for a single COVID-19 convalescent donor sample stimulated with SARS-CoV-2 pp A (blue arrow). The same donor sample stimulated with SARS-CoV-2 pp B clustered with the COVID-19 convalescent donor samples. Obtaining this clear separation of naïve and convalescent donor samples by chance is very unlikely, p < 2 -10 .

    Article Snippet: Curated SARS-CoV-2 peptide pools (130-127-309, Miltenyi and 3622-1, Mabtech) containing 9-22 mer peptides were used to activate SARS-CoV-2 antigen-responsive CD4 + and CD8 + T cells.

    Techniques: Marker